Seroepidemiologic study on SARS CoV2 infection in scholar children in Bogotá, Colombia

Some of the COVID-19 cases have been found to have no or only mild symptoms. Those asymptomatic cases can become a source of transmission of the virus, especially for vulnerable groups such as older adults or people with comorbidities. Due to that, it is important to estimate the real burden of the disease and to go beyond symptomatic cases.

Study population

We propose a cross-sectional study to estimate SARS-CoV-2 seroprevalence in children and adolescents between 6 and 18 years old attending educational institutions at the city of Bogotá, Colombia. It also seeks to explore factors associated with SARS-CoV-2 seropositivity and symptomatology.

Methodology

We designed a specific questionnaire for COVID-19 to collect information related to symptoms, exposure, and associated factors. Moreover, we are going to collect venous blood samples and to study the presence of antibodies to SARS-CoV-2 using a differential multiple serology. The diagnostic test is based on luminescence (Luminex) that would allow the detection of specific humoral immune responses in serum samples.

Analysis

We propose a descriptive analysis of the data according sex, age and age groups, exposure, and self-reported symptoms. We are going to calculate the proportion of seropositive individuals, and the proportion of symptomatic and asymptomatic cases. We will perform univariate, bivariate, stratified and multivariate analysis, and will calculate measures of association.

Insights

We consider that our results will have a significant impact on the knowledge of the epidemiology of SARS-CoV-2 in Colombia. It will provide information on the incidence of the virus in children and adolescents and will provide information on risk and protective factors.

Study Overview

Study nameSeroepidemiologic study on SARS CoV2 infection in scholar children in Bogotá, Colombia
ProjectSESCiB
Sources of Monetary or Material SupportHelmholtz Centre for Infection Research (HZI)
Region(s)Colombia
Location(s)Bogotá
Primary outcome(1) Seroprevalence of SARS-Cov-2, (2) Asymptomatic infection ratio, (3) Associated clinical, socioeconomic, and demographic factors.
Research Question(1) Estimate the proportion of scholar children and adolescents with identified IgG and IgM antibodies against SARS-CoV-2. (2) Determine the proportion of asymptomatic or subclinical infections in SARS-CoV-2 positive individuals. (3) Explore social, demographic, economic, and clinical factors that may be potentially associated with seropositivity to SARS-CoV-2 in children and adolescents.
Diagnostic test(s) used
  • Point-of-care test , Luminex Corp.
Antibody class(es)IgG, IgA
Study population N1000
Total population in the area1200000
Primary outcome(1) Seroprevalence of SARS-Cov-2, (2) Asymptomatic infection ratio, (3) Associated clinical, socioeconomic, and demographic factors.
Principal InvestigatorGérard Krause and Fernando Pio De La Hoz Restrepo , Helmholtz (HZI) and Universidad Nacional de Colombia , serohub@helmholtz-hzi.de
Team members
  • Gérard Krause, Principal Investigator, Helmholtz (HZI)
  • Fernando Pio De La Hoz Restrepo, Principal investigator, Universidad Nacional de Colombia
  • Tobias Kerrinnes, Co-principal investigator, Helmholtz (HZI)
  • Monika Strengert, Co-principal investigator, Helmholtz (HZI)
  • Johanna Carolina Torres Páez, Study Coordinator, Helmholtz (HZI) and Universidad Nacional de Colombia
  • Nathalie Verónica Fernández Villalobos, Study Coordinator, Helmholtz (HZI) and Universidad Nacional de Colombia
Period2020-09 till 2021-04
Study start2020-06
First results expected2020-11

Study metadata

{
   "abbreviation": "SESCiB",
   "draft": false,
   "iscjklanguage": false,
   "lastmod": "2020-07-22T16:10:00+02:00",
   "recruitment": {
     "country": [
       "Colombia"
     ],
     "group": "children",
     "location": [
       {
         "latitude": 4.624335,
         "longitude": -74.063644,
         "place_name": "Bogotá"
       }
     ],
     "type": "randomised individual invited voluntary"
   },
   "study_coord": {
     "email": "serohub@helmholtz-hzi.de",
     "institution": "Helmholtz (HZI)",
     "name": "Nathalie Verónica Fernández Villalobos",
     "role": "PhD student"
   },
   "study_dates": {
     "sample_end": "2021-04",
     "sample_start": "2020-09",
     "study_first_results": "2020-11",
     "study_start": "2020-06"
   },
   "study_info": {
     "diagnostic_test": [
       {
         "antibody_classes": [
           "IgM",
           "IgG"
         ],
         "company": "Luminex Corp.",
         "product": "Point-of-care test",
         "type": ""
       }
     ],
     "funding_sources": "Helmholtz Centre for Infection Research (HZI)",
     "primary_outcomes": "(1) Seroprevalence of SARS-Cov-2, (2) Asymptomatic infection ratio, (3) Associated clinical, socioeconomic, and demographic factors.",
     "research_question": "(1) Estimate the proportion of scholar children and adolescents with identified IgG and IgM antibodies against SARS-CoV-2. (2) Determine the proportion of asymptomatic or subclinical infections in SARS-CoV-2 positive individuals. (3) Explore social, demographic, economic, and clinical factors that may be potentially associated with seropositivity to SARS-CoV-2 in children and adolescents.",
     "tested_antibody_classes": [
       "IgG",
       "IgA"
     ]
   },
   "study_numbers": {
     "population": 1200000,
     "study_population": 1000
   },
   "study_pi": {
     "email": "serohub@helmholtz-hzi.de",
     "institution": "Helmholtz (HZI) and Universidad Nacional de Colombia",
     "name": "Gérard Krause and Fernando Pio De La Hoz Restrepo",
     "role": "Professor"
   },
   "team": [
     {
       "email": "serohub@helmholtz-hzi.de",
       "institution": "Helmholtz (HZI)",
       "name": "Gérard Krause",
       "position": "Head of Department Epidemiology",
       "role": "Principal Investigator",
       "title": "Prof. Dr. med."
     },
     {
       "email": "fpdelahozr@unal.edu.co",
       "institution": "Universidad Nacional de Colombia",
       "name": "Fernando Pio De La Hoz Restrepo",
       "position": "Director Public Health Department, Faculty of Medicine",
       "role": "Principal investigator",
       "title": "Prof. MD. PhD"
     },
     {
       "email": "",
       "institution": "Helmholtz (HZI)",
       "name": "Tobias Kerrinnes",
       "position": "",
       "role": "Co-principal investigator",
       "title": "Dr."
     },
     {
       "email": "",
       "institution": "Helmholtz (HZI)",
       "name": "Monika Strengert",
       "position": "Head of Laboratory",
       "role": "Co-principal investigator",
       "title": "PhD"
     },
     {
       "email": "Johanna.Torres@helmholtz-hzi.de",
       "institution": "Helmholtz (HZI) and Universidad Nacional de Colombia",
       "name": "Johanna Carolina Torres Páez",
       "position": "PhD Student",
       "role": "Study Coordinator",
       "title": "MD. MSc. Sp Pediatrics. "
     },
     {
       "email": "",
       "institution": "Helmholtz (HZI) and Universidad Nacional de Colombia",
       "name": "Nathalie Verónica Fernández Villalobos",
       "position": "PhD Student",
       "role": "Study Coordinator",
       "title": "MSc."
     }
   ],
   "title": "Seroepidemiologic study on SARS CoV2 infection in scholar children in Bogotá, Colombia "
 }